Landscape Painting Assignment Example | Topics and Well Written Essays - 250 words

Landscape Painting - Assignment Example Thus romanticism style of art involved exotic, more authentic, familiar and distant modes in harnessing imagination power as portrayed in landscape paintings that were prepared during this period. Romanticism went beyond the confines of population, industrialization unlike it preceding periods, the classical period and medievalism. In fact, romanticism exalted subjectivism, imagination, irrationalism and individualism. The art was highly characterized and influenced by artists’ fascination by their passions, nature, their moods, their mental potentials and the heroes. On the other hand, the Impressionism movement thrived in France between late 1860s and 1890s. This movement was inclined to optical realism since it sought to relate art to visual experience and the effect of light on appearance of various objects. The form of art in impressionism involved transcription of the artists’ visual sensation towards nature, which was unconcerned with the actual depiction of the objects. The main ideas of impressionism were, a quick painting of a landscape could depict it actual physical appearance and that art is promoted by a naà ¯ve vision, free from influence by intellectual preconceptions which were realist traditions and naturalist traditions. In comparison, the romanticism form and style of art applied to landscape paintings is distinguishable from the impression form art employed in landscape painting. This is evident from a keen observation of the following paintings, soleil levant by Claude Monet.

Who Was CELIA Essay Example | Topics and Well Written Essays - 250 words

Who Was CELIA - Essay Example Celia participated also in singing contest where she won many of them. As she grew up, she realized that her career was in music and assumed that her music created awareness to the world about the Cuban culture and the happiness of living life to the fullest (Tatum, 1002). In 1947, Celia enrolled to study at the Cuban Conservatory of Music and was discovered by one of the most famous orchestras called music group La Sonora Matancera. In 1960s, Celia moved to United States where she became a citizen due to political changes in Cuba. A few years later, she married Pedro Knight who was a trumpet player in her group. Celia recorded and performed with many musicians and became the voice of Salsa. While performing, she would wear colorful clothing and bright makeup on her face. Also, her dancing was energetic like her voice. Celia died in 2003 as a result of brain cancer (Tatum, 1003). In conclusion, it is evident that Celia was the Queen of salsa. Also, she had the passion of music since her childhood. Also, Celia created awareness to the world about the Cuban culture and the happiness of living life to the

Human sexuality Essay Example | Topics and Well Written Essays - 250 words - 1

Human sexuality - Essay Example enarios and the brain ability to mould it self is similar to plastic’s ability to change shape according to elements and objects that contacts the plastic. According to Doidge, a child’s brain has the ability to change over time with changes in environment (Doidge 98). He further states that when children are experiencing adolescent period, they should not be labeled according to their current sexuality preference as this preference changes over time until finally a sexual orientation becomes permanent. The book further states that there is a direct link between culture and brain and brain experiences alterations due to culture and not due to genes. This means that when the brain comes in contact with certain cultural practices, it adopts it and the individual starts behaving accordingly. The concept that brain is adaptive to environment can help us achieve our aims and objectives and we can alter our brain according to our needs. In relation to human sexuality an indiv idual can control his/her sexuality. If the individual wants to be labeled as straight then he/she should come in contact with their opposite sex and this will adapt the brain towards that sex and sexual

Who Was CELIA Essay Example | Topics and Well Written Essays - 250 words

Who Was CELIA - Essay Example Celia participated also in singing contest where she won many of them. As she grew up, she realized that her career was in music and assumed that her music created awareness to the world about the Cuban culture and the happiness of living life to the fullest (Tatum, 1002). In 1947, Celia enrolled to study at the Cuban Conservatory of Music and was discovered by one of the most famous orchestras called music group La Sonora Matancera. In 1960s, Celia moved to United States where she became a citizen due to political changes in Cuba. A few years later, she married Pedro Knight who was a trumpet player in her group. Celia recorded and performed with many musicians and became the voice of Salsa. While performing, she would wear colorful clothing and bright makeup on her face. Also, her dancing was energetic like her voice. Celia died in 2003 as a result of brain cancer (Tatum, 1003). In conclusion, it is evident that Celia was the Queen of salsa. Also, she had the passion of music since her childhood. Also, Celia created awareness to the world about the Cuban culture and the happiness of living life to the

Pop culture Essay Example | Topics and Well Written Essays - 1500 words

Pop culture - Essay Example Video games has opened a new culture in the world of entertainment leading to the emergence of a new form of popular culture. It is recorded that the initial games used interactive electronic devices with various display formats. Some of the earliest examples of video games the Cathode ray tube Amusement Device that was filed for a patent on 25 January 1947 by Thomas T. Goldsmith Jr. and Estle Ray Mann. The two designers were deeply inspired by radar display tech that was made up of an analog device that allowed a user to control a vector-drawn dot on the screen to simulate a missile being fired at targets, which were drawings fixed to the screen. While there has been a great element of changes leading to ink split on video game culture, the actual definition of the term is often treated as common sense to many people. The unraveling of the discourses surrounding video game culture offers room for people to envisioned in the power dynamics involved in attributing certain characteristics to it, as well as naming it. This approach has implications for how video games are studied as well as how they are interlinked with how culture is studied more broadly. By critically examining how video game culture has been defined in both press and academic articles, this paper seeks to elaborate how this cultural aspect has emerged to be considered on the most advanced forms of the popular culture. According to Tom Bissell in Extra Lives: Why video games matter, the rise of video in the current generation has resulted to the emergence of a new form of popular culture. He admits his great love and passion for the games. It is recorded that millions of adults all over the world, spend several hours every week playing video games leading to the growth of the industry. It is estimated that the industry is likely to outdo the film industry in Hollywood. However, the

How effectively does Shakespeare use scene 1 Essay Example for Free

How effectively does Shakespeare use scene 1 Essay A pair of star-crossed lovers take their life this immediately tells the audience the tragic fate of the lovers mentioned in the first 6lines of the prologue. Warning the audience of the sorrow and heartbreak that the play will lead to. Shakespeares Romeo and Juliet is based on the hatred between two families, the Montagues and The Capulets and how their anger and passion towards each other lead to the death of a beloved child from each family. Shakespeare uses Act 1, scene1 to introduce many of the main themes that appear throughout the play, these main themes are; Disorder, fate, light and Darkness, love and passion. Using this prologue Shakespeare is able to tell the audience the entire story line without them even watching the play. The line of the prologue beginning A pair of star-crossed lover. tells the audience straight away of the fate of the two characters involved. This set the scene for an inevitable ending whilst warning the audience what to expect, this is also back up throughout the play because as soon as one good thing beings disasters follows closely behind. Using prologues at the beginning of his plays, Shakespeare was able to capture and engage the audiences attention and quieten them down before any of the main characters are introduced. The takes place in the form of a sonnet, this would have contained the classic characteristics of love poetry, this would have been a very successful way to introduce the play because not only would it have captured the audiences attention and quietened them down, but Shakespeare would have used this prologue to give the audience vital information that they would have needed to understand the rest of the play. Shakespeare would have also been able to give the audience some idea of what to expect throughout the rest of the play. This was very effective as it used tight rhythm and rhyme designed to draw the audience in. The prologue at the beginning of Romeo and Juliet beings Two House-holds, both alike in dignity, in fair Verona (where we lay our scene) and continues Whose misadventured piteous overthrows, Do with their death bury their parents strife so in these two short lines Shakespeare has told his audience who the characters are going to be and where the play is set. Romeo and Juliet is set in Verona, Italy. Shakespeare chose this location because in the 16th century, Italy was regarded as a beautiful country, known for it wealth and romance, using these images and scene he had created in his head Shakespeare thought Verona would be the perfect place for extravagant lovers Verona was one of the 4 most important states in Italy. Each of these states has its own ruler; these rulers would have been very competitive, trying to gain superiority over others. Shakespeare used this key theme in the play Romeo and Juliet in the form of the Prince. The Prince would have ruled the state and overseen all activities that take place, this would include quarrelling and feuding between any families that live in the state. Shakespeare picked this up in his version of Romeo and Juliet making the Prince the ruler and the Montagues and the Capulets the two feuding families. Shakespeares Romeo and Juliet was not a piece of William Shakespeares direct work; he had based all the ideas and storylines on a book he had read by Arthur Brooke, entitled The Tragic History and Romeus and Juliet written in 1562. The History of this particular story goes back even further, to a Greek author names Xenophon. Whilst re-writing this particular storyline Shakespeare decided he was writing the play for different reasons to the ones Brook had used. Arthur Brook wrote the poem to warn young people of the dangers of physical attraction Shakespeare wrote his version because he was more interested in considering the validity of true love. Although Shakespeare had copied the story from two other people he had changed it and added pieces to make it his own. For example, Marcutio, Benvolio and the nurse were not featured in Xenophon version or Arthur Brooks version. Shakespeare had to do this because his audience would have known the original storyline and may have thought of Shakespeare as a fake, audiences in this time period would not have held back if they did not like the play they were being shown they would leave and cause havoc in the theatre. Not only did Shakespeare add characters, he added little parts of scenes to give the play more of an impact and reinforce the storyline. For example he added; a street fight, domestic scenes of food preparation, an incident with the musicians, Tybalts challenge to Romeo and the killing of Paris in Act5 scene 3. Act 1, Scene 1 opens with Capulet servants, Sampson and Gregory talking aggressively and violently in a busy market place looking for trouble, and therefore women being the weaker vessels are ever thrust to the wall, therefore I will push Montagues men from the wall, and thrust his maids to the wall. The two servants them speak of the hatred and bitterness between to two families whilst discussing violent and crude images. This section also represents the high-lightered issue of violence, which occurs in different sections of the play. Not only is this section crude and vulgar, it shows how violent these men maybe towards women and man. Its not long before a servant from the Montague family enters the scene, an argument sparks and the two sides are soon shouting at each other. This shows the audience of the loyalty that the servants have towards their households. When the argument/ fight continue the audience are shown that everyone is willing to put their life on the line for the sake of their family name. This is also observed later on in the play when Romeo and Juliet realise for the first time they are from 2 different, arguing families. This argument prepares the audience for other quarrels and brawls further on in the play. What, drawn and talk peace? I hate the word, As I hate hell, all Montagues, and thee. Have at thee, coward this particular dialogue spoken by Tybalt not only reflects the aggressiveness of his personality but tell the audience of the hate and bitterness between the two families. Shortly following these events Benvolio enters, Part, fools! Put up you swords, you know not what you do this shows the audience the peacekeeper in the play, the man that attempts to separate the two families and stop the brewing fight/argument. When this does not work Lord and Lady Capulet enter, followed by Lord and Lady Montague. Both the Capulet and Montague Lords want to join in the fight but are stopped by their wives, who tell them that they are too old. As the wives hold the pair back the Prince enters with his men. The Prince is seen as the figure of authority in the play; the person who controls all problems and is seen as someone who is capable of sorting out any feuding and arguments. He uses this power in a long speech where he talks of past quarrels between the Montagues and the Capulets and how it cannot continue. He does this to tell his audience that these arguments are not a one off thing and happen regularly disrupting the people of Verona. The two families recognise the figure of authority and part, this shows the audience that the Prince is superior to all other characters. The Prince continues, If ever you disturb our streets again, your lives shall pay the forfeit of the peace this is the only time that the Prince is seen as a threatening figure in the play, warning all members of the Capulet and the Montague household that if ever a fight breaks out again someone will pay the price with their life. The Princes language in this section is firm but at the same time, Shakespeare makes sure his audience wont view him as a threat to any of the other characters although he is. The Prince speaks in blank verse, to show he is superior and powerful and emphasises his intelligence. All the characters understand the Princes speech, but Romeo goes on the break the rule and is later banished from Verona although Romeo himself is not present when the fighting breaks out. This separates him from the rest of the characters making him seem detached from the families feuding and willing to carry on with his life without starting trouble every time he sees a member of the Capulet household. The scene continues, the Montagues leave with the Prince and the Capulets are instructed to return to their homes, Montague, Lady Montague and Benvolio are left in the market place, here a conversation takes place about Romeo and how he seems withdrawn from all goings on. Benvolio is asked to investigate into why Romeo is behaving like this and the rest of the Montagues leave. This is the first time the audience are introduced to Romeo when he begins his conversation with Benvolio. This is where the themes of love and passion are brought into the play. With Cupids arrow, she hath Dians wit; And in strong proof of chastity well armed, From Loves weak childish bow she lives uncharmed Romeo is speaking in verse to tell the audience about his love for Rosaline but Benvolio is not convinced that Romeo is actually in love, Romeo complains because the woman he claims to love will not marry nor love him back. Benvolio tells Romeo that other women will come along and he should try hard to forget about her, Romeo listens to this although really he is not convinced. Romeo uses and elaborate way of speaking Why then, O brawling love, O loving hate, O any thing of nothing first create! but this indicates to the audience his emotions are artificial, not coming from the heart. The audience would now know that Romeo is truly infatuated with Rosalind and that it was not just a fling or something he wasnt taking seriously. Throughout Romeos long speech to Benvolio he uses many oxymorons to emphasise what he thinks he is feeling. Loving hate and cold fire are perfect examples of when Romeo confuses himself with the overwhelming emotions he claims to have. The line love sickness, indicates to the audience that Romeo is almost infected with love for Rosaline and this may not be such a good thing. This would be showing the audience that love can to do terrible things to normal people, confusing the mind and turning and normal life chaotic. The light and Dark imagery is shown in the speech between Benvolio and Romeo but this is not mentioned until the audience is introduced to Juliet, Juliet is seen to be the light and Rosaline is seen to be the darkness that fills and confuses Romeos heart. This is where Shakespeare cleverly uses imagery to represent the two female characters in Romeos point of view. The other themes are continued throughout the play, from when Romeo and Juliet meet to when the both die together in the chapel of rest, where Juliets body has been placed. The theme of love however is resisted in Act 1 scene 5, when Romeo and Juliet fall in love and know they shouldnt. The idea of courtly love is present through most of this scene, although Romeo and Juliet are not experiences this, the theme and ides is the same. The idea of courtly love originally came from a European tradition from the middle ages but still known in Elizabethan times. This was basically a set of rules/ expectations of people who fall in love. These rules stated a number of things this included her coldness inflames the passion. He is consumed with melancholy and makes up verses about love this is present in Romeo and Juliet towards the end when Romeo is desperate to see Juliet and in his last hours makes up several verses to calm himself and prepare himself to meet his wife, Juliet in heaven. The idea of marriage also originated in Europe and is also present in the play, this theme is only mentioned by Capulet once, when he is talking to Paris about his arranged marriage to Juliet. Juliet was pleased and honoured to have been asked to marry Paris until she meet Romeo, this is when she began to resent the arranged marriage. Capulet tells Paris the way to win his daughters heart, the themes of love and passion, light and dark and violence are continued throughout. Shakespeare very cleverly uses Act 1 Scene 1 to introduce these theme either through the prologue, what the first characters say or the actions and imagery that are used. Shakespeare was able to take all of this into account and still produce his own version of Brooks poem The Tragic History and Romeus and Juliet.

The Biopharmaceuticals An Overview Biology Essay

The Biopharmaceuticals An Overview Biology Essay Biopharmaceuticals are defined as proteins and nucleic acids which are used for therapeutic purpose. These are high molecular weight substances prepared by biotechnological means and genetically engineered. Due to their complex structure and intricate molecular composition it is very difficult to characterize and formulate such products. Most of these products are derived from biological species and genetically engineered to produce a potent compound [1-3]. Humulin (biosynthetic recombinant human Insulin) was the first biopharmaceutical product developed by Genentech but licensed and marketed by Eli Lily and Company in 1982. In the modern era biopharmaceutical products have become a major facilitating reason of technological changes in health care sector, causing extraordinary benefits for patients, cost challenges for consumers, and profits for market shareholders.[4] Over the last decade biopharmaceutical market is the fastest growing segment in the pharmaceutical organizations. After post-recession period vigorous advancement has been seen in this area. It has been reported that approximately 40% of total pharmaceutical RD investment is occupied by biopharmaceutical products amongst which, monoclonal antibodies and product produced by recombinant DNA method are the most prevalent one.[5,6] Proteins with different amino acid sequences are the primary structure of such products. For determining the biological activity of a protein it is necessary to study its three-dimensional structure. The structure of protein is generally divided into four categories i.e. primary, secondary, tertiary and quaternary (fig. 1). The primary structure is the sequence of amino acid residues which folds and stabilized by the formation of hydrogen bonds with subsequent molecules in the polypeptide chain to produce an  ÃƒÅ½Ã‚ ± helix,  which is a spiral, rod-like st ructure, or a  ÃƒÅ½Ã‚ ² sheet,  a planar structure. This structure resembles to the secondary structure of proteins. The tertiary structure is a three-dimensional arrangement which gives an overall conformation of a polypeptide chain. Through hydrophobic interactions between the non-polar side chains and disulfide bonds, this tertiary structure is stabilized. A quaternary or multimeric protein contains two or more polypeptide chains, held together by non-covalent bonds.[7] fig 1. Structure of protein. Because of their unique physicochemical characteristic it is very hard and challenging to formulate biopharmaceuticals as products with therapeutic effects and storage characteristics. Meanwhile their formulation is in many ways different from conventional low-molecular drug formulation. Despite of such facts biopharmaceuticals gains lots of attention in the modern era of drug formulation. In the recent reviews it has been discussed that around 400 biotechnology-based medicinal and therapeutic agents are either registered or in the clinical trials are reviewed by the regulatory bodies. These agents are helpful in treating many life threatening diseases some of them are cancer, infectious diseases, autoimmune diseases, and AIDS/HIV. Some of the agents which are already in the market are monoclonal antibodies, recombinant hormones, cytokines, vaccines, recombinant blood factor, and therapeutic enzymes.[5] Discussion Biopharmaceutical market In 1970, discovery of recombinant DNA technology and monoclonal antibodies lead to initiate the development of biopharmaceutical industries. A substantial growth from $8 billion in 1992 to $149 billion in 2010 quenched the thirst of investors towards the biopharmaceutical industry. It is expected to reach $239 billion by 2015 with a growth rate of 9.9% annually. There are more than 400 Biotech drugs and vaccines are scrutinized under clinical stage, targeting more than 200 diseases. Meanwhile a large number of the population is now taking benefits from a number of biotechnological innovations like recombinant protein-based products e.g. monoclonal antibodies and some large molecule prescription drugs, which include hormones and vaccines. Table 1 illustrates some of the leading biopharmaceutical therapy classes by size worldwide in 2002. In 2008 approximately 39%, among the top 10 drugs sold globally, are biopharmaceuticals and is expected to rise to 79% of the top 10 drug sales by 20 14.[8] Table.1: Leading biopharmaceutical therapy classes by size worldwide 2002. Source: IMS MIDAS December 2002. However after the post recession period, fundings over health care and other sector is reduced which impede the growth rate of pharmaceutical industries up to significant extent. Although biopharmaceutical global sales thrive to attain high numbers in the global health sector. Biotechnological based products have generated $229 billion revenue in 2012 with an annual growth rate of 10.4%. Meanwhile the major focus of pharmaceutical organizations is on biopharmaceutical vs. Small molecule drugs which enables to allocate high funding towards this area. This may be due to very less generic biosimilar products available in the market. In year 2009 there are total 18 biopharmaceutical products are approved by US FDA which is higher than the previous years i.e. 11 in 2008 and 10 in 2007. In a report of Battelle Technology Partnership Practice, it was estimated that the overall impact of biopharmaceutical sector on the US economy is $917 billion. These companies are making alliances with oth er essential services such as computing and information, clinical trial and regulatory support services, technologies, manufacturing and equipment, and transportation and logistics providers.[9-11] Types of Biopharmaceuticals Cytokines Cytokines are a diverse group of non-antibody intercellular signalling regulatory proteins i.e. glycoprotein and low molecular weight peptides produced in very little amount inside the body. They function as a mediator for communication between various cells and induce certain response by binding to specific cell surface receptors which results in triggering various intracellular signal transduction mechanism. There are over 80 sub class of cytokines have been found. Some of these are categorized in table 2. They play an important role in coordinating embryonic development, cell growth and maturation, wound repair and healing, the immune response including acute phase reactions and septic shock, and new blood vessel formation. Cancer is among the major class of disease which is thoroughly scrutinized by the researchers with an emphasis on cytokines. However cytokines also play a vital role in mediating signalling response to systemic inflammation which tends to regulate specific neur onal network to induce sickness. Some animal studies reveal the role of cytokines in situations characterized by abnormal sleep, with emphasis on the pathogenesis of symptoms and consequences of OSAS (Obstructive sleep apnoea syndrome). Cytokines interact with specific cells via (1) intracrine, in which both cytokine and receptor remain within the cell (rare); (2) autocrine, where a cytokine acts on the receptors of the same cell it is produced; and (3) paracrine, where the cytokine from one cell type interacts with receptors on other cell types.[12-16] Table 2: categorization of cytokines. There are various cytokines products available in the market and some of them are reviewed under the clinical phase for example Interleukin-2 (IL-2) aldesleukin (Proleukin), for treating renal cell carcinoma is approved by FDA. IL-1 blocker, anakinra (Kineret) and rilonacept (Arcalyst) are used for the treatment of rheumatoid arthritis and cryopyrin-associated periodic syndromes respectively.[1] Enzymes Enzymes are complex molecules produced by living cells. They increase the rate of chemical reaction without even showing their own effect in the process and hence act as a catalyst in living organisms.[17] Because of their reaction specificity, catalytic efficiency and ability to operate under mild condition of temperature and hydrogen ion concentration they are preferred as a novel class of therapeutic agents. Despite of their therapeutic role enzyme endures significant importance as a replacement therapy in many genetic disorders. However there are some disadvantages as well associated with their use such as, their parental administration required extensive purification to remove contaminants. They are also very costly to prepare and due to their large molecular structure they have limited distribution in the body. As they are proteins hence when administered inside the body act as antigen to the host cell.[18,19] Enzymes are categorized under six major classes i.e transferases, lysases, hydrolases, isomerases, ligases, and oxidoreductases. The potential utility of enzyme as pharmaceutical was noted many decades ago. However most of the enzyme therapies are used to treat functional defects. Activase ® (alteplase; recombinant human tissue plasminogen activator), is the first recombinant enzyme drug which was approved by the Food and Drug Administration in 1987. Pulmozyme ® (Dornase alpha), a recombinant DNAse I that digests DNA in the mucous secretions in lungs, used to treat cystic fibrosis. Adagen ® (pegadamase bovine), is the first marketed product as enzyme therapy for the treatment of SCID (severe combined immunodeficiency disease).[20] Hormones Hormone is characterized as an important group of regulatory molecule which is synthesized in an organ or in tissue and then secreted into circulatory system where it affects respective organs whose cell bears an appropriate receptors. They are chemical messengers either small or a large macromolecule and are fairly defined as endocrine hormones. Approximately all endocrine hormones possess therapeutic importance and hence subsequently used by the researchers e.g. insulin(Insugen ®, Humulin ®, Novolin ®), human growth hormone (Ascellacrin ®, Crescormon ®), glucagon, and the gonadotrophins.[1] Antibodies: Antibodies or immunoglobulin are proteins produced, as an immune response, through white blood cells when a foreign particle or antigen intrudes inside the body. There are five classes of immunoglobulin IgE, IgM, IgG, IgD, and IgA. Amongst them IgG is the most common as a biopharmaceutical agents. A more specified term i.e. monoclonal antibodies (mAb  or  moAb) are the novel class compounds that are first developed in mid 1970s by kohler and Milstein, who successfully fused immortal cells with antibody- producing B lymphocytes. This fusion results in a hybrid which is stable, cancerous, antibody- producing cells. These are referred as hybridoma cells which unveil great source of monoclonal antibody. mAb identify antigens on a particular foreign body very specifically. mAbs are categorized under four groups according to their source: murine (derived solely from mice), chimeric (mixture of murine and human sequences), humanised (contain over 90% human sequences) and fully human.[8, 17] In 1986, Murobmonab (OKT3), a murine antibody, is the first monoclonal antibody tested as a therapeutic in humans. However it fails to produce a substantial response in the transplantation rejection patients because of production of anti-murine antibody.[20] Monoclonal antibodies are the fastest growing therapeutic class in the field of biopharmaceutical market. The market of monoclonal antibodies crosses the mark of $30 billion in 2011. Infliximab (Remicade) is the top selling monoclonal antibody in 2002 which was developed by Centocor and is marketed by Centocor and Schering-Plough for the treatment of rheumatoid arthritis (RA) and Crohns disease. Rituximab (Rituxan), developed by Genentech and IDEC and marketed by Genentech, IDEC and Roche, for the treatment of non-Hodgkins lymphoma, is the second largest selling monoclonal antibody.[21] Vaccines: Vaccines are antigenic proteins which are either partial or modified form of bacteria, viruses and toxins which causes certain diseases against which vaccine protects. The antigenic property of vaccines is altered from its original form by certain processes such as utilizing attenuated viruses, inactivated viruses, part of a virus or bacteria and toxin produced by bacteria; hence they lack ability to cause disease although, endures ability to produce an immune response.[22] Vaccines are the only known prophylactic approach which are commonly available to cure many infective diseases. Vaccines cover the global market share of $ 3 billion annually. Government health agencies run different immunization programs which alleviate the risk of many infective disorders. However, there are approximately 15 million people die every year from certain infectious diseases that can be cured by vaccination process. Pharmaceutical and biopharmaceutical industries seek great potential in this area but the use is limited because of their high production cost, delivery and distribution problems. Vaccines may contain animal derived products such as Bovine serum albumin or fetal calf serum or human derived product for example using cell lines of human tissues. However in recent years transgenic and recombinant plants are also utilized for the production of vaccines.[12,23,24] There are different vaccines available in the market for many infectious diseases such as poliomyelitis, cholera, diphtheria, tetanus, hepatitis B, hepatitis A, measles, meningitis etc. For hepatitis B virus some examples are Baraclude (Entecavir ®), Adefovir dipivoxil (Hepsera ®), Lamivudine (Epivir ®-HBV, 3TC), Alfa Interferon (Intron ® A, Infergen ®, Roferon ®).[1,25] Antisense drugs: Antisense compounds are non-coding strand of messenger RNA(mRNA), which hybridize with mRNA and inactivates the ability of genes to produce the protein for which it holds the recipe.[26] They do not alter human gene causing variation in human genetic information. It was first developed in 1978 using a string of DNA nucleotide which binds with messenger RNA and block its function. These drugs are designed to treat a wide range of diseases such inflammatory, infectious, cancer and cardiovascular diseases. Fomiversen was the first antisense drug approved in 1998 for the treatment of cytomegalovirus retinits in AIDS patients. These are categorized under three sub-classes i.e. antisense oligonucleotides(ASONs), ribozymes and RNA interference(RNAi). Affinitak, manufactured by ISIS pharmaceuticals and Eli Lilly, is used for the treatment of non-small-cell lung carcinoma(NSCLC). There are certain antisense compound are under review by health agencies for their therapeutic efficacy towards ma ny anomalies[27,28] Peptide therapeutics: Peptides are polymers having one or two amino acids linked with amides and are chemically synthesized by using liquid phase or solid phase synthesis. Their chemical synthesis is preferred over biosynthetic approach because it facilitates the conjugation or incorporation of small molecules and non-natural amino acids. This approach reveals the potential of such molecules as cytotoxic agents for antitumor therapy. Peptides with non-natural opens up the possibility for greater chemical diversity, analogous to small-molecule medicinal chemistry approaches for developing high-af ¬Ã‚ nity, highspeci ¬Ã‚ city molecular recognition. Cationic antimicrobial peptides (CAMPs) and peptidoglycan recognition proteins (PGRPs)are developed from the effectors of innate immune response in eukaryotes during their defense mechanism. However there are not many products of peptides found in the market but researchers are trying to develop some therapeutic peptides and proteins with significant effect towards certain diseases. Nesiritide (Natrecor, Noratak) is produced by Johnson Johnson2001, Teriparatide (Forteo) is developed and marketed by Lily in 2002 Enfuvirtide (Fuzeon) is manufactured by Trimeris 2003.[29-31] Challenges associated with biopharmaceutical products: Biopharmaceutical products such as monoclonal antibodies, vaccines, certain hormones, and cell therapies are rewarded as some of the most promising innovation in the field of medicine and health. However there are certain risk and challenges associated with such products which hamper their performance. One of the major challenges is the purification of proteins although it can be achieved by various processes but still purification of such compound is very critical hence requires a lot of attention during manufacturing. Next generation pharmaceutical.[32] However the whole process is carried out under two phases i.e. upstream and downstream regulation. Immunogenicity associated with some of these proteins is also a major concern for biopharmaceutical agents. A repeated injection of human protein may affect the immune tolerance towards self- antigen, leading to humoral response.[33]   Biopharmaceutical products require greater attention, process level as well as monitory level, in the development phase of the drug discovery process it requires approximately $200 million to develop a biopharmaceutical product hence the risk associated with their failure during development stage is increased. Apart from purification and immunogenicity some other issues also make some impact during their development such as government regulations which influence the cost of such products. As discussed earlier it takes a large amount of money to develop such agents hence companies also compensate their development cost and earn profits from their products, ones marketed, which exponentially increases the cost of the drugs. Last and one of the most important risk associated with such products is the introduction of biosimilar products. Biopharmaceutical market is growing very fast, the initial compounds of the first generation are now coming to an end. The expiry of the patents opens the market for generic products which not only influence the established market of some very popular brands, it also forms a milieu of competition to sell the products at cheaper rates which is compensated by their quality.[34] Conclusion Biopharmaceutical products are one amongst the novel class of therapeutic compounds. These are medical agents obtained from biological sources and some special one are manufactured by biotechnological means. They are cellular products, therapeutic proteins, vaccines, gene therapy products and plasma blood product derivatives. Erythropoietins, insulins, and monoclonal antibodies, are some of the leading biopharmaceutical products available in the market hence biopharmaceutical companies are now focusing on their use in long-term conditions such as cardiovascular disease, diabetes and asthma. The blockbuster sales of some existing biopharmaceutical products accompanied by the tremendous innovation and a plethora of pipeline products demonstrate the vigorous growth in the biopharmaceutical market in upcoming years. This significant growth is supported by some of the key segments of the field like monoclonal antibody and vaccines. In addition approaches like gene therapy and peptide ther apeutics holds enormous potential for some life threatening anomalies and the combined approach of proteomics, genomics and biotechnology is predicted to empower the most part of the healthcare sector in the upcoming days.